Memes

Save/Kill The Animals
Originating with Super Metroid, the meme refers to a long running Bid War on GDQ to either rescue or leave behind the animals during Super Metriod's escape sequence. This became a vitally important decision to GDQ Viewers, and donation comments often reflect this during the entire marathon.

"That's never happened before!"
Often uttered when things go wrong on stream or in games.

WAH
Starting in SGDQ 2016 with ProtoMagicalGirl's run of Super Mario Land, ending during the Super Metroid run of 2017 when it stood in front of the shuttle bus.

POYO
Associated with commentator Edobean to speak with a cutesy Kirby voice.

"Greetings from Germany"
A common joke during donations

Hype Trains
Money donations made to meet an incentive

No Fun Allowed
Something about GDQ trying to become more professional and advertiser friendly

Twitch Chat
Because of GDQ's Twitch Chat becoming subscriber only, there have been many chants to "Free the Plebs" (those not subscribed). "RUINED" "RESET"

Dance Dad
"Dance Dad" is a regular staple of Games Done Quick live events starting with AGDQ 2019. He is usually seen in between games when the camera feed is on the audience, and he can be seen dancing to the intermission music. He is known as wolfman2000 online, and has commentary on Games Done Quick on his Twitter and Twitch channels.

Prizes
Sent

Lamp
With the SGDQ 2020 event hosted online, prize segments were shown in host Sent's mother's living room. Among the decor was a Tiffany Lamp, which the audience quickly noticed and commented about. Every prize segment the Twitch chat would be filled with comments regarding the lamp. Shortly after the staff noticed the popularity of the lamp, they created a Twitch emote for the channel of the lamp.

Poetry/Haiku
During SGDQ 2020, donators became creative with their donations and many participated by delivering their comments in the forms of poems and haikus. Popular subjects of the poems were about the charity Doctors Without Borders, Games Done Quick, or the game that was running at the time.

HONK
The protagonist of Untitled Goose Game, - an independently produced video game - became a viral sensation and made their feathery, chaotic presence known at AGDQ 2020 with many donations reading, in some part, HONK.

Orb
Orb came from SMW/SMB3. Some runners say it when collecting the orb you use to complete certain levels in these games. the meme probably started with Runner grandPOObear. During AGDQ 2019 it was first mentioned during the Super Mario Bros. 3Mix run and started it's true legacy during Semblance's run by halfcoordinated. Since then, the meme has been a staple at every single GDQ event, "ORB" being yelled by the crowd every time a orb shaped object shows up during a run.